As being the 16S profiles within the gut show up incredibly different for intracellular and extracellular DNA [33], qPCR is expected to mirror the NGS-focused community framework both equally quantitatively and qualitatively additional closely than circulation cytometry.
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We quantified total germs employing common bacterial primers [29] by qPCR in 114 adult fecal DNA samples that were analyzed for microbiota composition making use of Illumina MiSeq for 16S rRNA gene amplicon sequencing [14]. The qPCR threshold cycle (Ct) values were transformed into the estimates of bacterial genomes current in one g of feces for a proxy of full bacterial counts.
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